Full
Outline
To establish a method for the determination of glucosamine hydrochloride content in health foods, with phenyl-isothiocyanate as the derivatizing reagent by pre-column derivatization-high performance liquid chromatography (HPLC).
Samples were ultrasonically extracted with water, and the extract was derivatized with phenyl-isothiocyanate. Separation was achieved on an Agilent Poroshell 120 EC-C18 column (4.6 mm×150 mm, 4 μm) via gradient elution with sodium acetate and methanol as mobile phases at a flow rate of 0.8 mL/min.Detection was performed at 245 nm using a HPLC-ultraviolet detector. Pre-column derivatization conditions: 1 mL sample solution was vortex-mixed with 6 mL phosphate buffer, equilibrated at room temperature for 15 min, then mixed with 2 mL phenyl-isothiocyanate reagent in the same colorimetric tube. The mixture was heated in an 80 ℃water bath for 30 min, rapidly cooled to room temperature, and diluted to 10 mL with water.
Glucosamine hydrochloride exhibited a good linearity in the range of 25–250 mg/100 g (r2=0.9993). The limit of detection for glucosamine hydrochloride was 0.0050 mg/mL, and the limit of quantitation was 0.0100 mg/mL. The repeatability relative standard deviation was 0.81%, and the recovery rates ranged from 97.9% to 99.9%.
This method is simple to operate and exhibits good repeatability, making it suitable for the determination of glucosamine hydrochloride content in health foods.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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