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Outline
To optimize the preparation process of Ziziphi Spinosae Semen peptide-zinc chelate(ZSSP-Zn) and characterize its structure.
This study optimized the preparation of ZSSP-Zn used a combination of single-factor experiments and response surface methodology. The structures and compositions of ZSSP-Zn were characterized using ultraviolet spectroscopy, Fourier transform infrared spectroscopy, amino acid analysis, scanning electron microscopy and size distribution. The stability of the ZSSP-Zn was investigated through in vitro simulated gastrointestinal digestion.
The optimal chelation conditions for ZSSP-Zn included peptide-to-zinc ratio of 3:1 (m:m), pH 6, chelation time of 2 h and a chelation temperature of 60 ℃. Under these conditions, the chelation rate was (41.97±0.82)%. After chelation, the ultraviolet absorption peak shifts to the blue.Amino nitrogen atoms, carboxyl oxygen atoms, carbonyl oxygen atoms and hydroxyl oxygen atoms served as key binding sites for zinc ions. Chelation led to formation of loose granular structures with reduced particle size. The in vitro simulated gastrointestinal digestion analysis indicated that the ZSSP-Zn chelate exhibited good stability.
The optimized ZSSP-Zn preparation process in this study is stable and reliable, with a more uniform particle size distribution of the chelate complex. These results provide technical references for the development of traditional Chinese medicine-based zinc supplements.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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