Full
Outline
To develop a rapid detecting heavy metal mercury and lead residues in Asparagus officinalis L. by latex microsphere immunochromatographic test strips.
Heavy metal mercury and lead antibodies were conjugated to latex microspheres. Nitrocellulose membranes were coated with heavy metal mercury and lead antigens as the test line (T line) and goat anti-mouse antibody as the control line (C line). After optimizing conditions, sensitivity, specificity and stability were evaluated, followed by testing Asparagus officinalis L. samples.
The optimal antibody concentration for mercury detection was 40 μg/mL, with an optimal coating antigen mass concentration of 0.5 mg/mL.optimal probe volume of 3 μL, optimal detection pH of 7, minimum limit of detection 0.28 ng/mL, half-inhibition rate of 5.60 ng/mL and quantitative range of 1.00–50.00 ng/mL. For lead detection, the optimal antibody concentration was 40 μg/mL, with an optimal coating stock mass concentration of 0.5 mg/mL, optimal probe addition volume of 4 μL, optimal detection pH of 6, minimum limit of detection was 0.29 ng/mL, half-inhibition rate of 2.15 ng/mL and quantitative range of 0.10–10.00 ng/mL. Both latex microsphere immunochromatographic test strips demonstrated good specificity and could be stored for over one year.
This study successfully develope a rapid test strip for heavy metals mercury and lead based on latex microsphere immunochromatography technology. It exhibits high specificity and sensitivity, making it suitable for on-site screening of mercury and lead residues in Asparagus officinalis L..
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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