Full
Outline
To investigate the contamination status, toxin genes carriage patterns, antimicrobial resistance phenotypes and genomic evolution patterns of Clostridium perfringens in commercial meat and meat products in Ma’anshan City.
A total of 300 meat and meat product samples were collected from 3 districts, 3 counties and online shopping channels in Ma’anshan City from 2021 to 2024. Clostridium perfringens was identified by isolation culture and polymerase chain reaction (PCR). The 7 kinds of toxin genes were detected by multiplex fluorescence PCR.The antimicrobial susceptibility profiles of 12 kinds of antibiotics were determined by agar dilution method.Whole-genome sequencing (WGS) combined with bioinformatics analysis was used to perform multilocus sequence typing (MLST), antimicrobial resistance gene prediction and core-genome phylogenetic tree construction.
The total positive detection rate of Clostridium perfringens in 300 samples was 18.67% (56/300). There were statistically significant differences in the detection rates among different years, sample types, sampling regions and sites (all P<0.05), among which raw poultry meat (45.16%) and farmers’ market samples (30.82%) were the most heavily contaminated. The detection of 7 kinds of toxin genes (cpa, cpb, iap, etx, cpe, netB, cbp2) showed that the positive rate of cpa gene was 100.00%, and the positive rates of cpe and cbp2 were 7.14% and 48.21%, respectively. The strains were dominated by type A (92.86%), and type E (1.79%) and type F (5.36%) were also detected. Antimicrobial susceptibility testing showed that the strains had high resistance rates to tetracycline (64.29%) and clindamycin (32.14%) and 26.79% of the strains exhibited multidrug resistance; the susceptible rates to cefoperazone, meropenem and chloramphenicol were 100.00%. Whole-genome analysis revealed that the 56 strains contained 50 kinds of MLST, including 26 kinds of newly discovered types. A total of 9 kinds of antimicrobial resistance genes were identified and the carriage rates of mprF, tetA(P), and tetB(P) ranked the top 3 (85.71%, 73.21% and 69.64%, respectively). The core-genome phylogenetic tree indicated genetic homology among strains from different years and regions, suggesting a risk of cross-regional transmission.
The contamination of Clostridium perfringens in commercial meat and meat products in Ma’anshan City is relatively severe, with high toxin gene carriage rates, prominent multidrug resistance and genetic diversity. There are risks of cross-regional transmission and food poisoning, so it is necessary to strengthen the supervision of raw poultry meat and farmers’ market links.
8
2
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
关闭全屏
No. 86 Xueyuan South Road, Haidian District, Beijing
010-62662699(Operation and Technical Support)
qkjq@cast.org.cn
Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House
