Full
Outline
To evaluate the improvement effect of Lactobacillus gasseri GMLCA-Lac 1 on dextran sulfate sodium (DSS)-induced ulcerative colitis in mice and its potential mechanism of action.
The strain characteristics were analyzed through whole-genome sequencing, and the changes in disease phenotype, intestinal barrier function and immune indicators were evaluated in combination with animal experiments.
The genome of Lactobacillus gasseri GMLCA-Lac 1 comprised a circular chromosome and 2 plasmids, encoding 1980 predicted open reading frames. Strain-specific genes were significantly enriched in glycolysis/gluconeogenesis, pyruvate metabolism and antioxidation pathways, which conferred robust capacities for lactic acid production, short-chain fatty acid synthesis and intestinal stress tolerance. In animal experiments, Lactobacillus gasseri GMLCA-Lac 1 exerted a dose-dependent attenuation of DSS-induced body weight loss, diarrhea and hematochezia, reducing the disease activity index, restoring colon length and ameliorating colonic pathological damage. It also promoted intestinal mucus barrier repair by increasing goblet cell density. At the same time, Lactobacillus gasseri GMLCA-Lac 1 significantly reduced the levels of pro-inflammatory cytokines in serum, included interleukin-1β (IL-1β), interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α), lowered the spleen index and helped restore the balance of immune function.
Lactobacillus gasseri GMLCA-Lac 1 alleviates colitis in mice through the synergistic effects of repairing the intestinal mucosal barrier and modulating inflammation and immunity, providing experimental evidence for its potential as a candidate strain for microecological therapy of inflammatory bowel disease.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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