Full
Outline
To assist the extraction of polysaccharides from Cistanche deserticola by high-speed shear technology, deeply investigate the optimal combination of process parameters, and evaluate the in vitro antioxidant capacity of the extracted polysaccharides.
Using Cistanche deserticola from Alxa as the raw material, a high-speed shear-assisted extraction technique was employed to extract polysaccharides. The extraction process was optimized using single-factor experiments and response surface methodology. The in vitro antioxidant activity of the polysaccharides was evaluated by measuring their scavenging rates against 1,1-diphenyl-2-picrylhydrazine (DPPH)radicals, hydroxyl radicals and 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) cationic radicals, as well as their total antioxidant capacity determined by the ferric reducing antioxidant power (FRAP) assay.
The optimal extraction process conditions were as follows: a solid-liquid ratio of 1:30 (g:mL), a shearing speed of 10000 r/min, a shearing time of 30 s and a shearing frequency of 3 times. Under these optimized conditions, the actual average yield of Cistanche deserticola polysaccharides was 2.47%±0.15%, showing essentially no difference from the predicted value(2.32%). The results of in vitro antioxidant experiments indicated that Cistanche deserticola polysaccharides exhibited strong scavenging abilities against different types of free radicals and demonstrated a clear dose-effect relationship within specific concentration ranges.
The high-speed shearing-assist extraction process for Cistanche deserticola polysaccharides, optimize via response surface methodology, demonstrates both stability and feasibility. The extract polysaccharides exhibit notable in vitro antioxidant activity, providing a robust theoretical foundation for their further development and application in functional foods and pharmaceutical industries.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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