Full
Outline
To screen lyophilization protectants for Lactiplantibacillus plantarum DPA1C and develop an efficient composite lyoprotectant formulation.
A basal lyoprotectant was first established to ensure a certain survival rate of the freeze-dried bacterial powder. On this basis, single-factor experiments were conducted to identify lyoprotectant components with significant effects on the freeze-drying survival rate of the strain. Subsequently, orthogonal experimental design was employed to optimize the ratio of the selected single-factor protectants.
The final formulation contained 12% skim milk, 9% trehalose, 3% exopolysaccharide produced by Lactiplantibacillus plantarum DPA1C and 2% sodium glutamate. Under these conditions, the survival rate of Lactiplantibacillus plantarum DPA1C reached 95.26%±2.01%, markedly higher than those with single protectants or control groups. This demonstrated a notable synergistic protective effect against lyophilization damage.
The exopolysaccharide produced by Lactiplantibacillus plantarum DPA1C is incorporated as one of the components of its own lyoprotectant, and an optimized composite protectant that significantly enhances the strain’s resistance to freeze-drying stress is obtained. This work provides a data reference for the industrial production of highly active bacterial preparations, and also offers a reference for the application of exopolysaccharides produced by other lactic acid bacteria.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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