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Rapid detection of matrine residues in Fragaria ananassa Duch by colloidal gold immunochromatography
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Ai-Hong WU1, Qian-Qian ZHANG2, Shun-Yao ZHOU3, Zheng LI1, Hui-Min ZHANG1, Li-Qiang LIU1, *, Chuan-Lai XU1
Journal of Food Safety & Quality | 2026, 17(1) : 61 - 67
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Journal of Food Safety & Quality | 2026, 17(1): 61-67
• Special Topic: Rapid Detection Technology for Food Safety •
Rapid detection of matrine residues in Fragaria ananassa Duch by colloidal gold immunochromatography
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Ai-Hong WU1, Qian-Qian ZHANG2, Shun-Yao ZHOU3, Zheng LI1, Hui-Min ZHANG1, Li-Qiang LIU1, *, Chuan-Lai XU1
Affiliations
  • 1.School of Food Science, Jiangnan University, Wuxi 214000, China
  • 2.Wuxi Binhu District Agricultural Technology Extension Station, Wuxi 214000, China
  • 3.Wuxi Binhu District Agricultural Comprehensive Administrative Law Enforcement Brigade, Wuxi 214000, China
doi: 10.19812/j.cnki.jfsq11-5956/ts.20250925004
Outline
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Objective

To establish an analytical method for the rapid detection of matrine pesticide residues in Fragaria ananassa Duch by colloidal gold immunochromatography.

Methods

By optimizing the labeling parameters of gold nanoparticles and the concentration of the test strip detection line, colloidal gold could bind with monoclonal antibodies against matrine under optimal conditions. By utilizing highly specific recognition of antibodies and antigens and visualization of gold labeled antibodies, the localization detection of matrine pesticide residues in Fragaria ananassa Duch could be achieved.

Results

Determine the labeling parameters as follows: 1 mL of colloidal gold solution with 20 μL of 0.1 mol/L K2CO3, 8 μg of antibody, 5% polyvinyl pyrrolidone (PVP) surfactant,and an antigen transmembrane concentration of 0.10 mg/mL; using the above conditions, the limit of detection of matrine in Fragaria ananassa Duch samples was set to 10 μg/kg, and rapid detection was performed on 126 collected Fragaria ananassa Duch samples.

Conclusion

This method has high sensitivity and specificity, and can quickly and accurately detect residual matrine in samples, enabling rapid screening of a large number of samples on site.

matrine  /  Fragaria ananassa Duch  /  immunochromatography  /  colloidal gold
吴爱红, 张倩倩, 周顺瑶, 李征, 张慧敏, 刘丽强, 胥传来. 胶体金免疫层析法快速检测草莓中苦参碱残留. 食品安全质量检测学报, 2026 , 17 (1) : 61 -67 . DOI: 10.19812/j.cnki.jfsq11-5956/ts.20250925004
Ai-Hong WU, Qian-Qian ZHANG, Shun-Yao ZHOU, Zheng LI, Hui-Min ZHANG, Li-Qiang LIU, Chuan-Lai XU. Rapid detection of matrine residues in Fragaria ananassa Duch by colloidal gold immunochromatography[J]. Journal of Food Safety & Quality, 2026 , 17 (1) : 61 -67 . DOI: 10.19812/j.cnki.jfsq11-5956/ts.20250925004
Appendix
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Year 2026 volume 17 Issue 1
8
2
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Article Info
doi: 10.19812/j.cnki.jfsq11-5956/ts.20250925004
  • Receive Date：2025-09-25
  • Online Date：2026-11-05
Article Data
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History
  • Received：2025-09-25
Funding
Affiliations
    1.School of Food Science, Jiangnan University, Wuxi 214000, China
    2.Wuxi Binhu District Agricultural Technology Extension Station, Wuxi 214000, China
    3.Wuxi Binhu District Agricultural Comprehensive Administrative Law Enforcement Brigade, Wuxi 214000, China
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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