Full
Outline
To establish a method for the determination of 5 kinds of nucleotides in aquatic products by high performance liquid chromatography.
After optimization of chromatographic conditions and selection of pretreatment conditions, the sample was extracted twice with 5% perchloric acid solution at 4 ℃, and then extracted with 0.01 mol/L KH2PO4 (containing 1.45 mmol/L tetrabutyl ammonium bisulfate,pH=5.0)-acetonitrile (95:5, V:V) was eluted by mobile phase, separated by C18 (250 mm×4.6 mm×5 μm) column,detected by ultraviolet detector, and quantified by external standard method with standard curve.
The 5 kinds of nucleotides had a good linear relationship in the range of 0.1–100.0 μg/mL, and the correlation coefficient was greater than 0.999. The limits of detection and limits of quantitation were 1.0–2.0 mg/kg and 3.0–6.0 mg/kg,respectively, and the precisions were 2.00%–6.87%. The spike recoveries were 92.3%–105.6%, the intra-assay standard deviations were 0.63%–7.90%, and the inter-assay standard deviations were 1.72%–7.34%.
This method has high sensitivity and good repeatability, and can be used for the qualitative and quantitative analysis of 5 kinds of nucleotides in aquatic products.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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