Full
Outline
To establish a method for the determination of 7 kinds of organic solvent residues in tea polyphenols by headspace-gas chromatography (HS-GC), and conduct risk analysis.
A method for simultaneous determination of 7 kinds of organic solvent residues (n-hexane, methanol, dichloromethane, acetone,ethanol, trichloromethane and ethyl acetate) in tea polyphenols was established using a 50% N,N-dimethylacetamide solution as the matrix, separated by a DB-23 capillary column (60 m×0.32 mm, 0.25 μm), and detected by a flame ionization detector (FID).
HS-GC could effectively separate 7 kinds of solvents to be tested, with good intra day and inter day precision. The standard curve showed a good linear relationship within the concentration range under investigation, with limit of detection of 0.03–1.71 μg/g and limit of quantitation of 0.12–5.77 μg/g. At the same time, residue testing and risk analysis were conducted on 27 batches of tea polyphenol samples in the market.According to the regulations of Chinese Pharmacopoeia, methanol, ethyl acetate, n-hexane and ethanol were detected in some tea polyphenol samples. Among them, 33.33% of the samples had n-hexane and 14.81% of the samples had ethanol exceeding the standard. However, through the analysis of permissible daily exposure (PDE), the health risks caused by n-hexane and ethanol were relatively low.
This research method is simple, accurate, and suitable for the determination of 7 kinds of residual organic solvents in tea polyphenols.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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