Full
Outline
To establish a method for the rapid determination of malathion drug residues in Lonicera japonica by colloidal gold immunochromatography.
The residual malathion in honeysuckle can bind to the colloidal gold-labeled monoclonal antibody, inhibiting the binding of the antibody to the antigen on the nitrocellulose membrane (T line) and resulting in a color change on the T line, this study analyzed the effects of various factors, such as pH, antibody addition amount, antigen coating amount, type of freeze-drying protective agent, and Tween 20 addition amount on the test results.
The optimal conditions determined were as follows: The optimal pH for preparing the malathion monoclonal antibody (mAb) and goat anti-mouse immunoglobulin (lgG) gold-labeled antibody was 9.0; the optimal amount of Tween 20 added was 50 μL; and the optimal lyophilization protectant for the gold-labeled antibody was a mixture of 1.0 g sucrose, 0.5 g bovine serum albumin and 0.5 g mannitol. The optimized method achieved a limit of detection was 50 μg/kg for malathion in Lonicera japonica. Furthermore, the method exhibited high specificity for malathion; no cross-reactivity was observed with isocarbophos, profenofos, phoxim or fenthion at concentrations ranging from 200 to 1000 μg/kg.Method validation using negative and positive samples showed a false-positive rate of 1% and a false-negative rate of 0.5%, achieving consistency with the results from existing standard methods. The test strips produced based on this method could be stored for one year at room temperature under sealed and dry conditions.
The method established in this study is simple to operate, highly specific, sensitive and accurate. It requires a short detection time and demonstrates good stability, making it suitable for the rapid screening of malathion residues in large batches of Lonicera japonica samples.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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