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Outline
To establish a method for the rapid determination of the content of 9 kinds of aconitum alkaloids in homemade Radix aconiti Kusnezoffii medicinal wine by ultra performance liquid chromatography-tandem mass spectrometry.
The wild Radix aconiti Kusnezoffii in Yunnan was used as the main raw material. It was made into 5 kinds of different shapes (whole, peeled, flake, block, powder), and 250 g was soaked in 900 mL of 52-degree corn to brew liquor, and the soaking time was about 1.5 years. Took 1 mL sample of medicinal liquor, added 30% methanol water solution (3:7, V:V) to 100 mL, and filtered it with 0.22 μm filter membrane, ready for testing. C18 column and mobile phase of acetonitrile-0.1% formic acid water were used for gradient elution separation, multi-reaction monitoring mode detection, and quantification was carried out by matrix working curve external standard method.
The linear relationship of 9 kinds of aconitum alkaloids was good in the range of 1–100 μg/L, the correlation coefficients were all greater than 0.999, the limits of detection were 0.63–0.98 μg/L, the limit of quantitation were 2.11–3.26 μg/L, and the recovery rates were 81.14%–116.01% at low,medium and high concentrations, the relative standard deviations were 0.97%–11.26% (n=6). According to the analysis of actual samples, the content of diester alkaloids of 9 kinds of aconitum alkaloids in medicinal liquor was high, among which aconitine had the most content and strong toxicity.
This method is simple in pretreatment, high in sensitivity, good in accuracy and high in detection efficiency. It is suitable for rapid screening of 9 kinds of aconitum alkaloids in Radix aconiti Kusnezoffii homemade medicinal liquor in health emergency poisoning events, and provides strong technical support for rapid diagnosis of medical treatment and corresponding emergency treatment measures.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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