Microorganisms in food, especially pathogenic microorganisms, have caused serious effects on food safety and human health. In recent years, the occurrence of mild and severe infectious diseases caused by pathogenic microorganisms has attracted people’s attention. Hence, the realization of real-time analysis and detection of pathogenic microorganisms in food is of great significance to ensure food safety and human health. As an emerging detection technology, microfluidic technology has been widely used in the rapid detection of pathogenic microorganisms in food due to its advantages of miniaturization, integration and automation. At the same time, its high throughput, high sensitivity, high portability and repeatability detection also show great potential in practical applications. To this end, this paper summarized the advantages and disadvantages of different types of materials,including inorganic, polymer, paper-based, composite and hydrogel materials, as well as their practical applications on microfluidic chips, secondly reviewed the application progress of microfluidic technology in the detection of different pathogenic microorganisms (including bacteria, fungi, parasites and viruses) in food in the past 5 years. Finally,summarized and prospected the shortcomings and challenges of microfluidic technology in practical application detection, aiming to provide reference for the development of microfluidic technology in the field of food safety testing.
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |