To analyze the effects of different termination methods on the yield of theaflavins.
Theaflavins were prepared through the enzymatic action of potato polyphenol oxidase (PPO). A high performance liquid chromatography (HPLC) analysis was conducted to detect theaflavin compounds. The effects of 6 kinds of different termination methods on theaflavin yield were then analyzed.
Following treatment with microwave medium power, microwave high power, a 100 °C water bath and 3 kinds of inhibitors, the concentration of theaflavins was measured. The results of the HPLC analysis indicated that the 100 °C water bath treatment resulted in the highest theaflavin content, with theaflavin-3,3’-gallate (TFDG) reaching 32.47 µg/mL and theaflavin substances at 119.12 µg/mL.
The concentration of theaflavins is highest after treatment in a 100 °C water bath.The research findings may provide valuable reference for rapid detection and industrial-scale production of theaflavins.
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |