Full
Outline
To establish a method for quantitative determination of 2 kinds of novel tadalafil derivatives in blended wine, coffee and table candy by ultra performance liquid chromatography-tandem mass spectrometry.
Waters HSS T3 (2.1 mm×100 mm, 1.8 μm) reversed-phase chromatographic column was used for gradient elution with 0.1% formic acid water and acetonitrile as the mobile phase, and was detected by mass spectrometry with multiple reaction monitoring.
In the mass concentration range of 1.0–20.0 ng/mL, there was a good nonlinear relationship between 2 kinds of new tadalafil derivatives, diethylaminopretadalafil and dipropylaminopretadalafil (r2>0.99); the limits of detection of the method were all 0.05 mg/kg and the limits of quantitation were all 0.10 mg/kg. The average recovery rate range at the 3 levels of 1, 2 and 10 times limits of quantitation were 82.5% to 95.6%, and the relative standard deviation were 1.9%–7.8% (n=6). The method had good accuracy.
This method is simple, accurate and sensitive, and can be used for the accurate and rapid determination of the content of diethylaminopretadalafil and dipropylaminopretadalafil in blended wine, coffee and table candy.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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