Full
Outline
To establish a method for the simultaneous detection of benzoic acid, sorbic acid and dehydroacetic acid in pre-made meals by high performance liquid chromatography.
Sodium hydroxide solution was added to the sample for ultrasonic extraction. Protein was precipitated with potassium ferrocyanide and zinc acetate solution. Separation was carried out using an Agilent XDB-C18 (4.6 mm×250 mm, 5 μm)chromatographic column. The mobile phase was ammonium acetate solution and methanol (93:7, V:V), with a flow rate of 1 mL/min and a column temperature of 30 ℃. The detection was carried out by a diode array detector with detection wavelengths of 230 nm and 293 nm. At the same time, the spectra of the standard solution and the sample solution were collected respectively by a diode array detector. By comparing the spectra of the target peak in the standard and sample solutions, the samples were further qualitatively confirmed.
The 3 kinds of preservatives had a good linear relationship within the range of 0.5–100.0 mg/L, with correlation coefficients (r)greater than or equal to 0.9994. The limits of detection were 1.7 mg/kg, the limits of quantitation were 5.0 mg/kg, the recovery rates were 92.0%–104.0%, and the relative standard deviations were 0.1–1.6%.
This method is simple, rapid, has good reproducibility and high recovery rate, and is suitable for the rapid detection of 3 kinds of preservatives, namely benzoic acid, sorbic acid and dehydroacetic acid in pre-made meals.
10
2
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
关闭全屏
No. 86 Xueyuan South Road, Haidian District, Beijing
010-62662699(Operation and Technical Support)
qkjq@cast.org.cn
Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House
