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To optimize ultrasonic assisted enzymatic extraction of antimicrobial peptides from Carassius auratus scales.
In this study, the diameter of inhibition zone against Escherichia coli and peptide recovery rate were used as evaluation indicators. The enzymatic effects of pepsin, trypsin, papain, and neutral protease on Carassius auratus scales were compared and analyzed to screen the optimal enzyme for enzymolysis.Through single-factor experiments, the effects of substrate concentration, enzyme activity, enzymolysis time, and ultrasonic power on the extraction efficiency of antimicrobial peptides from crucian carp scales were systematically analyzed. On this basis, three key factors (enzyme activity, enzymolysis time, and ultrasonic power) were selected for orthogonal optimization experiments.
Pepsin was identified as the optimal enzyme for enzymolysis. Under the enzymatic hydrolysis process with a substrate mass concentration of 200 mg/mL, enzymatic hydrolysis time of 4 h,ultrasonic power of 120 W, and enzyme activity force of 5000 U/g, the antibacterial effect of the obtained antimicrobial peptide was the best, with a inhibition zone diameter of (2.66±0.66) cm against Escherichia coli and a short peptide recovery rate of 22.52%±1.64%.
The ultrasonic assisted enzymatic extract of antimicrobial peptides from Carassius auratus scales provides a critical theoretical foundation for subsequent separation and purification.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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