Full
Outline
To establish a quantitative analytical method for 17 kinds of triterpenic acid compounds in Poria cocos and investigate their content in samples from different regions.
A high performance liquid chromatography method was developed using an C18 column with gradient elution (acetonitrile-0.1% phosphoric acid solution as the mobile phase). The flow rate was set at 1.0 mL/min, column temperature at 20 ℃, and injection volume at 20 μL. The 17 kinds of triterpenic acid compounds were quantified by wavelength switching mode, and the obtained data were subjected to analysis.
All 17 kinds of compounds exhibited good linearity (r>0.999) within their respective concentration ranges. The spike recovery rates ranged from 80.9% to 92.5%, with relative standard deviations below 5.0%. The limits of detection and limits of quantitation respectively were 0.343–2.839 μg/mL and 1.143–9.462 μg/mL.
The established high performance liquid chromatography method features simple sample pretreatment, high sensitivity, excellent stability and accuracy, making it suitable for the determination of 17 kinds of triterpenic acids in Poria cocos. This study provides a foundational basis for the development and utilization of functional components in Poria cocos.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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