To evaluate the contamination levels of Bacillus cereus and Burkholderia gladioli pathovar cocovenenans (BGC), along with the moisture content and dehydroacetic acid levels in wet rice and flour products in Yunnan Province.
From July to December 2024, a total of 108 samples of bulk wet rice products (including 48 portions of vermicelli, 47 portions of rice noodles, etc.) were collected from prefectures in Yunnan Province, covering markets (50.93%), supermarkets (17.59%), small restaurants (12.96%) and other locations. Pathogens were detected in accordance with GB 4789.14—2014 National food safety standard food-Microbiological examination-Detection of Bacillus cereus and GB 4789.29—2020 National food safety standard food-Microbiological examination-Detection of Burkholderia gladioli (formerly Pseudomonas cocovenenans subsp. Farinofermentans), while moisture and dehydroacetic acid content were determined in accordance with GB 5009.3—2016 National food safety standard-Determination of moisture in food and GB 5009.121—2016 National food safety standard-Determination of dehydroacetic acid in food.
The detection rate of Bacillus cereus was 31.48% (34/108), with a quantitative range of <10–6.8×104 CFU/g (P90=4140 CFU/g). The highest detection rate was observed in roll noodles (43.75%,P=0.022), and the detection rate in refrigerated samples (45.71%) was significantly higher than that in the room temperature group (P<0.001). Neither BGC nor excessive dehydroacetic acid was detected in any of the samples. The 3 samples of wet rice noodle products failed to meet the moisture content requirements stipulated in DBS53/017—2023 Local food safety standards of Yunnan Province-Rice noodles, rolled rice noodles and rice vermicelli (blocks).
Wet rice products in Yunnan are at risk of contamination by Bacillus cereus, particularly roll noodles and refrigerated products. It is essential to strengthen hygiene control in both production and circulation processes. No BGC contamination or potential risk of food poisoning caused by rice-fermenting bacteria has been identified.
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |