Full
Outline
To establish a method for determination the content of functional components of various medicinal and edible homology (glycyrrhizic acid, kaempferol-3-O-rutinoside, isoflavan glucoside,luteolin-7-glucoside, puerarin, chlorogenic acid, salidroside and glycyrrhizin) in botanical beverages by ultra-high pressure liquid chromatography.
The appropriate amount of botanical beverage sample was weighed, the sample was vortex-dispersed with a methanol-water solution and then performed ultrasound-assisted extraction to fully release the target dispersed components. After centrifugation, the supernatant was collected. An EC-C18 chromatographic column was used for the separation of various components in the extract, and quantitative analysis was performed using the external standard method., the content of 8 kinds of efficacy components in the botanical beverage was simultaneously detected.
The 8 kinds of active components exhibited good linearity within the mass concentration range of 0.50–25.00 mg/L, with correlation coefficients (r)>0.998. The limits of detection and limits of quantitation for the 8 kinds of functional components ranged from 0.05 to 0.50 mg/kg and 0.16 to 1.50 mg/kg,respectively. The recovery rates were between 83.2% and 96.1% and the relative standard deviations of the test results were 3.2%–6.2% (n=3).
The established method demonstrates excellent precision and recovery rates,along with satisfactory sensitivity, making it suitable for determining the content of these 8 kinds of pharmacologically active components in botanical beverages.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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