To optimize the extraction process of Polygonatum kingianum, and determine the optimal process parameters under the premise of ensuring high antioxidant activity.
This study utilized the entropy weight method to ascertain the weight coefficients of various metrics, including extract yield, total polysaccharide content and antioxidant activity, facilitating the calculation of a comprehensive score. The effects of ethanol volume fraction, solid-liquid ratio, extraction duration and extraction temperature on the extraction process were examined through single-factor experiments. Based on the above, the extraction process of Polygonatum kingianum was optimized using response surface methodology, with the comprehensive score serving as the response value.
The optimal extraction conditions were an ethanol volume fraction of 60%, an extraction temperature of 90 ℃, an extraction duration of 2 h and a solid-liquid ratio of 1:40 (g:mL). By using the determined optimal process parameters, the 3 parallel batches of samples yielded comprehensive scores of 98.92, 99.09 and 98.81, with a relative standard deviation of 0.14%.
This research optimize an extraction process of Polygonatum kingianum,confirming its feasibility and providing a basis for the development and utilization of Polygonatum kingianum.
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |