Full
Outline
To demonstrate the feasibility of using methyl β-orcinol carboxylate as a marker for identification of “tech cong” tea，and establish its detection method.
A detection method for methyl β-orcinol carboxylate in tea was established by using QuEChERS technology for sample preparation and gas chromatography-mass spectrometry for determination. The content of methyl β-orcinol carboxylate in teas and Yunnan Ramalina fastigiata was analyzed. Common teas were scented with Ramalina fastigiate by simulating the counterfeiting technique of “tech cong” tea. Subsequently，the content analysis of methyl β-orcinol carboxylate and sensory evaluation were carried out on the scented teas.
Methyl β-orcinol carboxylate was identified as a characteristic component of lichen-based fragrances like oakmoss extracts and treemoss extracts，not a natural constituent of genuine old-bush tea leaves. It could be transferred from Yunnan Ramalina fastigiata to ordinary tea leaves through scenting，imparting an “old-bush” flavor. A total of 31 samples labeled “old-bush Shuixian” purchased online were tested，and the detection rate of methyl β-orcinol carboxylate was 58.1%.
Methyl β-orcinol carboxylate can serve as a marker for identifying “tech cong” tea. The gas chromatography-mass spectrometry method combined with QuEChERS sample preparation can detect the content of methyl β-orcinol carboxylate. This method can be applied to the authenticity identification of “old-bush Shuixian” tea，which is of great significance for strengthening the supervision of the tea market and cracking down on the illegal counterfeiting of high-grade tea.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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