Full
Outline
To establish a method for the determination of trace benzo(a)pyrene in water by ultra performance liquid chromatography-atmospheric pressure chemical ionization-tandem mass spectrometry(UPLC-APCI-MS/MS).
Sample pretreatment was performed using solid-phase extraction with cyclohexane as the elution solvent. Gradient elution was carried out with acetonitrile-water as the mobile phase at a flow rate of 0.6 mL/min, and an injection volume of 10 μL. Separation was achieved on an HPLC column Shim-pack GIST C18.Detection was performed in positive ion mode by APCI with multiple reaction monitoring.
Methodological validation was conducted under optimized conditions, demonstrating a good linear relationship for benz(a)pyrene within the mass concentration range of 0.50–70.0 μg/L (r2=0.9992). The limit of detection was 0.484 ng/L and a limit of quantitation was 1.940 ng/L. The 3 different levels of spiked samples were applied to drinking water, surface water and groundwater, respectively, yielding an average spiked recovery rate of 86.9%–93.5% with relative standard deviations ranging from 0.5% to 4.9%.
This method is easy to operate, highly efficient in detection, accurate in data and exhibits high sensitivity, making it suitable for the determination of trace benz(a)pyrene in drinking water and source water.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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