Full
Outline
To systematically characterize the long-term evolutionary patterns of nutritional quality and metabolic profiles in a single Oryza sativa L. cultivar.
Oryza sativa L. samples of the cultivar Ezhong No.5, preserved by the Hubei Academy of Agricultural Sciences, encompassing 5 production years (2009 and 2019–2022), were analyzed. Under conditions of identical cultivar background, consistent cultivation management, and traceable storage conditions, cooking quality, intrinsic viscosity and major nutritional components were determined using conventional physicochemical methods, while comprehensive metabolic characterization was conducted through untargeted metabolomics based on ultra performance liquid chromatography-tandem mass spectrometry.
A total of 273 metabolites were identified. According to the Classy Fire chemical classification system, these metabolites could be divided into 9 superclasses, with lipids and lipid-like molecules accounting for the largest proportion. Oryza sativa L. samples from different production years exhibited stage-dependent variations in amylose, gel consistency, protein and amino acids. Principal component analysis demonstrated a distinct year-dependent separation of metabolic profiles. Differential metabolites were primarily enriched in fatty acyls, glycerophospholipids, flavonoids and organic acid derivatives, indicating that lipid metabolism and secondary metabolic pathways contributed substantially to year-related metabolic variations. A gradient boosting machine model established based on metabolomic profiles exhibited favorable discriminative performance, achieving an accuracy of 93.33% in three-fold cross-validation, thereby demonstrating the presence of stable and distinguishable metabolic signatures among Oryza sativa L. samples from different production years.
During long-term storage and chronological progression, the quality traits and metabolic composition of the same Oryza sativa L. variety exhibit stable and identifiable differences over time. This study, based on a scarce sample of the same variety spanning more than ten years, reveals the chronological evolution of Oryza sativa L. quality and metabolic characteristics, providing an important reference for Oryza sativa L. quality evaluation and storage management research.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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