Full
Outline
To establish a method for the simultaneous determination of 46 kinds of antibiotic residues including 22 kinds of sulfonamides, 15 kinds of quinolones, 9 kinds of nitroimidazoles in animal muscle tissues by ultra performance liquid chromatography-tandem mass spectrometry.
The samples were extracted by 90% acetonitrile aqueous solution, purified by PRiME HLB solid-phase extraction column, concentrated by nitrogen blowing and converted to solvent, and determined by ultra performance liquid chromatography-tandem mass spectrometry. The mobile phase consisted of 0.1% formic acid in water and acetonitrile, with gradient elution.Detection was performed in multiple reaction monitoring mode, and quantification was carried out using the external standard method.
The 46 kinds of antibiotics showed good linear relations within their respective concentration ranges, with limits of quantitation ranging from 1-5 μg/kg. The average recovery rates were 60.2% to 106.8%, and the relative standard deviations were 0.2% to 12.2%.
This method features high detection efficiency and high throughput, and can meet the rapid screening requirements of sulfonamides, quinolones and nitroimidazoles in animal muscle tissues.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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