Full
Outline
To establish a analytical method for detecting pesticide residues of 9 kinds of diamide insecticides in medicinal and edible homologous food by ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS).
In this experiment, 2.00 g of 4 kinds of medicinal and edible homologous food samples: Lycium barbarum, Fructus Mori, Chrysanthemum morifolium Ramat and Angelica sinensis were selected. The extraction solvent was 0.1% formic acid in acetonitrile, and the extraction procedure involved vortex oscillation for 5 min followed by ultrasonication for 10 min. The extraction salts used were 4 g magnesium sulfate+1 g NaCl+1 g C6H5Na3O7·2H2O+0.5 g C6H6Na2O7·1.5 H2O. The purification method employed 150 mg MgSO4, 50 mg C18 and 50 mg primary secondary amine (PSA) per milliliter of extract.
The results showed that within the range of 2.0–100.0 μg/L, the linear correlation coefficient of 9 kinds of diamide insecticides was greater than 0.999, the method developed in this study demonstrates excellent stability and repeatability. Limit of detection was 3.0 μg/kg and the limit of quantitation was 10.0 μg/kg. When the sample was spiked at levels of 10, 20 and 100 μg/kg, the recovery rates were 70.6%-96.0% and relative standard deviations were 3.4%-9.0% (n=6).
This study addresses the limitations of existing detection methods for diamide insecticide residues by developing a more comprehensive and effective analytical method. The improved methodology enhances the accuracy and reliability of pesticide residue detection in medicinal and edible homologous food, demonstrating significant practical value in ensuring the food safety of these dual-purpose items.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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