Full
Outline
To evaluate the applicability of the Soleris rapid microbial detection method for detecting molds and yeasts in food and its equivalence with the national standard method.
Validation experiments were performed in accordance with domestic and international standards, focusing on 5 core parameters: Inclusivity, exclusivity, sensitivity, relative limit of detection, robustness and inter-batch variability. A systematic equivalence comparison was conducted against the conventional reference method specified in GB 4789.15—2016 National food safety standard-Microbiological examination of food-Enumeration of molds and yeasts.
All 30 target mold and yeast strains were successfully detected, yielding an inclusivity rate of 100%; while none of the 31 non-target strains were detected, resulting in an exclusivity rate of 100%. The relative limit of detection values across diverse food matrices were consistently below the preset acceptance threshold of 1.5. Robustness and differences between reagent batches had no statistically significant impact on the detection results, demonstrating excellent repeatability. The detection time was shortened to within 48 hours.
The detection results of mold and yeast in food using the Soleris microbial rapid detection method are equivalent to the GB 4789.15—2016 method and can be used for the rapid detection of mold and yeast in food, providing efficient technical support for food safety quality control, supervision and sampling inspection, and emergency screening.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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