To improve the survival rate of probiotic cells and prepare microencapsulated compound probiotics.
In this study, microencapsulation technology was employed, using sodium alginate and skimmed milk powder as wall materials, and stachyose and konjac micro-powder as core materials to encapsulate probiotics. The effects of stachyose and konjac micro-powder on the survival rate and release rate of microencapsulated composite probiotics were investigated through room temperature storage and simulated gastrointestinal fluid treatment.
When the mass ratio of probiotics, stachyose, lactulose, isomalto-oligosaccharides and konjac micro-powder solution was 1:5:2:2:3, the probiotic composite microcapsules prepared in this way had a survival rate of 84.5% after being treated in gastric juice for 90 min, a release rate of 93.0% after being treated in intestinal juice for 90 min, a survival rate as high as 93.2% after being stored at room temperature for 30 d, and a survival rate as high as 90.0% after being stored at room temperature for 60 d.
The added stachyose, lactulose, isomalto-oligosaccharides and konjac micro-powder can increase the survival rate of microencapsulated probiotics during storage at room temperature, with good gastric acid resistance and excellent enteric effect.
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |