Full
Outline
To evaluate the degradation effects of Pseudoalteromonas carrageenovora A1 on Saccharina japonica and systematically analyze the polysaccharide characteristics of the degradation products.
A strain of Pseudoalteromonas carrageenovora A1 capable of degrading Saccharina japonica was obtained through screening. The fermentation broth acquired after Saccharina japonica fermentation was analyzed. The content determination, monosaccharide composition analysis and molecular weight analysis were performed on the fermentation broth and its fractional ethanol precipitation components, and the Saccharina japonica residues were observed by scanning electron microscopy.
This strain could significantly damage the cell wall of Saccharina japonica. The degradation products contained polysaccharides and oligosaccharides with a wide molecular weight range, while the supernatant mainly enriched oligosaccharides of 1.4–1.7 kDa. Further analysis of the precipitated components revealed that the content of fucose was relatively high, with 70% alcohol precipitation reaching 4.08%; the monosaccharide composition analysis indicated that the precipitation was rich in fucose, galactose and mannose, and the characteristic units of alginate were detected, confirming that the products contained sulfated fucopolysaccharides and alginate.
Pseudoalteromonas carrageenovora A1 has the function of decomposing Saccharina japonica, destroying the cell wall and releasing the carbohydrate compounds. Polysaccharides and oligosaccharides with different molecular weights from Saccharina japonica can be effectively obtained by alcohol precipitation, providing a method to develop kelp polysaccharides and oligosaccharides through fermentation technology and obtain bioactive materials for the food industry.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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