To explore the effects of cold oxygen plasma (COP) coupling ultraviolet (UV) technology on microbial inactivation in Spirulina platensis slurry, and optimize sterilization conditions based on parameters such as color and cell fragmentation.
Spirulina platensis slurry with varying mass fractions was processed by adjusting the power and time of COP-UV technology and measuring changes in total bacterial count, pathogenic bacteria count, color and protein solubility.
After comprehensively considering the sterilization efficiency, cost and algal cell rupture, the optimal sterilization conditions for COP-UV technology were as follows: 200 mL 20%(m:V) algal slurry with 800 W COP and 40 W UV for 5 min treatment, the maximum temperature was only 38 ℃, the sterilization efficiency of the algal slurry reached 95.66% and the dissolution rate of algal cell protein was only 10.56%, the total color of Spirulina platensis slurry showed only slight visible changes. In addition, the sterilization order of COP-UV technology on 3 kinds of pathogenic bacteria was: Bacillus subtilis (74.86%)>Escherichia coli(61.45%)>Staphylococcus aureus (58.53%), which might be attributed to the difference in the composition of the cell membrane and wall of pathogenic bacteria.
COP-UV technology can achieve effective sterilization with minimal structural damage to cells of Spirulina platensis, ensuring the safety and quality of Spirulina platensis powder and related products. Therefore, COP-UV technology will hold great potential applications in ensuring the quality and safety of heat-sensitive products.
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |