Full
Outline
To study the optimal application conditions for alginate lyase and construction the optimal process for a specialized composite enzyme preparation targeting Ascophyllum nodosum.
The optimal reaction conditions of alginate lyase were determined by the control variable method. Based on these optimal conditions, Ascophyllum nodosum was selected as the research material, and cellulase, pectinase and papain were used for composite enzyme preparation. The optimal technological parameters for compound enzymatic hydrolysis of Ascophyllum nodosum were obtained via response surface methodology. Under the optimized conditions, Ascophyllum nodosum from different geographical populations (Peru, Chile and France) were subjected to enzymatic hydrolysis. Using the extraction rate of alginate oligosaccharides as the evaluation index, the degradation capacity of the compound enzyme preparation against seaweed from different origins was analyzed. By comparing the extraction rate of alginate oligosaccharides and the solid conversion rate of enzymatic hydrolysis products, the hydrolysis efficiency of the composite enzyme preparation was verified.
The optimal application conditions for the alginate lyase were an enzyme dosage of 0.50%, pH 7.5, reaction time 18 h and hydrolysis temperature 45 ℃. The optimal process conditions for the composite enzyme preparation of Ascophyllum nodosum enzymatic hydrolysis were cellulase 1.82%, pectinase 1.2%, papain 1.5% and alginate lyase 0.6%, solid-liquid ratio was 1:25 (g:mL), with an enzymatic hydrolysis temperature of 45 ℃, pH 7.22 and a reaction time of 18 h. Under these conditions, the extraction rates of alginate oligosaccharides was 14.46%.
By analyzing the degradation effects of the composite enzyme preparation from this study on Ascophyllum nodosum from different sources, it is found that this composite enzyme preparation effectively degrades Ascophyllum nodosum from various sources, and its enzymatic efficacy surpasses that of commercially available composite enzyme preparations on the market. The composite enzyme preparation constructed in this study can directly enzymolyze Ascophyllum nodosum to prepare alginate oligosaccharides, realizing the direct utilization of seaweed resources. It provides a new idea and technical reference for the low-cost preparation of functional alginate oligosaccharides and the development of related agricultural biological products.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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