Full
Outline
To optimize the method for free astaxanthin detection and develop an extraction process for astaxanthin from Xanthophyllomyces dendrorhous powder.
By optimizing the detection time and mobile phase ratio, an analytical method for free astaxanthin based on high performance liquid chromatography (HPLC) was established. On this basis, using Xanthophyllomyces dendrorhous powder as raw material, the extraction process for astaxanthin from Xanthophyllomyces dendrorhous powder was developed via single-factor experiments, Plackett-Burman design, and response surface methodology, using astaxanthin extraction yield as the evaluation index.
The limit of detection was 0.55 μg/g, and the limit of quantitation was 0.16 μg/g. The spike recoveries ranged from 105.12% to 127.70%, and the relative standard deviation in the precision test was less than 0.14%. The extraction process for astaxanthin from Xanthophyllomyces dendrorhous powder was developed and optimized as follows: The average particle size of superfine Xanthophyllomyces dendrorhous powder was 0.55 μm;the water addition ratio was 3.30:1 (mL:g); the dimethyl sulfoxide (DMSO) addition ratio was 41.25:1 (mL:g); the acetone addition ratio was 137.50:1 (mL:g); the extraction was performed twice; after water addition, the mixture was incubated at 45 ℃ for 5 min; and after DMSO addition, it was incubated at 45 ℃ for 15 min. The extraction yield was 2.74 mg/g.
The established HPLC method for free astaxanthin is sensitive, accurate and reproducible, enabling rapid quantitative analysis of free astaxanthin in Xanthophyllomyces dendrorhous. The optimized extraction process is efficient, stable and environmentally friendly. This study provides technical support for the application of Xanthophyllomyces dendrorhous astaxanthin in aquaculture, food and other fields.
13
3
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
关闭全屏
No. 86 Xueyuan South Road, Haidian District, Beijing
010-62662699(Operation and Technical Support)
qkjq@cast.org.cn
Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House
