Full
Outline
To establish a method for simultaneous determination of the content of beauvericin (BEA), enniatin A (ENN A), enniatin A1 (ENN A1), enniatin B (ENN B) and enniatin B1 (ENN B1) in Castanea mollissima Blume by QuEChERS-ultra performance liquid chromatography-tandem mass spectrometry.
The mass spectrometry conditions for 5 kinds of emerging mycotoxins were optimized. The optimal chromatographic column and mobile phase conditions based on the separation degree and peak shape of the chromatographic peaks were selected. The best extraction reagent and QuEChERS purification column through recovery rate indicators were determined. The matrix effects of Castanea mollissima Blume samples on 5 kinds of emerging mycotoxins were analyzed. Finally, a methodological experiment was conducted on the established method.
The method ultimately used acetonitrile-water (80:20, V:V) as the extraction reagent, selected QuEChERS column (primary secondary amine 50 mg, MgSO4 150 mg) for cleaning Treatment. The ACQUITY UPLC BEH C18 column was used as the chromatographic column and acetonitrile-2 mmoL/L ammonium acetate was used as the mobile phase, the elution mode was gradient elution. Matrix effects of the method could not be ignored, the isotope internal standard method should be used for quantitative analysis. The limits of detection of BEA, ENN A, ENN A1,ENN B and ENN B1 were 0.03 μg/kg and the limits of quantatition were 0.1 μg/kg, the recovery rates were between 89.5% and 125.0%, the relative standard deviations were 1.6% to 8.4%, the correlation coefficients were all than or equal to 0.9990.
This method is simple, rapid, with the high accurates and ensitivity, which can be used for simultaneous determination of the content of BEA, ENN A, ENN A1, ENN B and ENN B1 in Castanea mollissima Blume.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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