To compare the analytical performance of high performance liquid chromatography with fluorescence detector and high performance liquid chromatography with diode array detector for determination of 9 kinds of biogenic amines in fermented wines.

Under identical pretreatment and chromatographic conditions, both methods were applied to determine 9 kinds of biogenic amines in fermented wines. A comprehensive evaluation was conducted based on linear range, sensitivity, precision, recovery and resistance to interference.

Both methods exhibited good linearity (r²≥0.9986) within the 1–50 μg/mL range, with precision (<3.00%)and accuracy meeting detection requirements. The fluorescence detection method exhibited higher sensitivity than the diode array detection method for most biogenic amines, with a detection limit as low as 0.05 μg/mL; however, its sensitivity for histamine, octopamine, and tyramine was comparable to that of the diode array detection method; The diode array detection method provided a more uniform response across different biogenic amines, whereas the fluorescence detection method demonstrates superior resistance to interference in complex matrices..

The high performance liquid chromatography-fluorescence detector is suitable for high-sensitivity detection of trace biogenic amines, whereas high performance liquid chromatography-diode array detector holds practical value for balanced screening and routine analysis of multiple biogenic amines.