Full
Outline
To establish a rapid method for the determination of tetracycline in honey by nanogold-aptamer colorimetric method.
Discovery Studio 2022 software was used to optimize the tertiary structure of aptamer and select the optimal adapter. The relationship between different tetracycline concentrations and changes in absorbance ratio at specific wavelengths was explored under optimized conditions.
Under the optimal conditions of AuNPs:aptamer:tetracycline:NaCl ratio of 5:1:1:1 (V:V:V:V), NaCl concentration of 0.4 mol/L,and adapter concentration of 0.30 mol/L, the established method showed a good linear relationship with the change in absorbance ratio within the range of tetracycline mass concentration of 1–200 ng/mL. The linear regression equation was Y=0.007X+0.16, r2=0.9906, and the limit of detection was 0.838 ng/mL. Specific analysis showed that this method had high specificity for tetracycline and had no color reaction with enrofloxacin, salbutamol and other drugs.The recovery rates of honey determination with added standards were 95.30%–97.96%. Compared with the detection results of GB/T 21317—2007 Method for detecting tetracycline residues in animal derived foods-Liquid chromatography-mass spectrometry/mass spectrometry and high performance liquid chromatography, it had good accuracy.
This method has strong specificity, is easy to operate, and can quickly quantify tetracycline residues in samples.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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