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Outline
To establish the optimal extraction process of total flavonoids from the perianth and pollen of Hippophae rhamnoides L. subsp. sinensis Rousi male flowers and compare their antioxidant activity.
The total flavonoids of perianth and pollen were extracted by ultrasonic-assisted alcohol extraction method. The content of total flavonoids was determined by spectrophotometry. The optimal extraction process of both was optimized by single factor experiment and response surface optimization. Additionally, in vitro experiments were conducted to evaluate the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and the 2,2’-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) cationic radical scavenging activities of total flavonoids from the calyx and pollen.
The optimal extraction process for the perianth was 60% ethanol by volume, a solid-to-liquid ratio of 10:1(mL:g), ultrasonic extraction at 61 °C for 39 min, under which the total flavonoid yield was (4.59±0.12)%. The optimal extraction process for pollen was 70% ethanol, solid-to-liquid ratio of 10:1 (mL:g), ultrasonic extraction at 52 °C for 62 min, under which the total flavonoid yield was (1.88±0.06)%. At a mass concentration of 4.0 mg/mL, the DPPH radical scavenging rates of total flavonoids from perianth and pollen were (84.87 ± 0.03)% and (82.53 ± 0.11)%,respectively. At a mass concentration of 0.80 mg/mL, the ABTS cationic radical scavenging rates of total flavonoids from perianth and pollen were (83.21 ± 0.13)% and (79.37 ± 0.06)%, respectively.
This study analyzes the differences in total flavonoid content and antioxidant capacity between the perianth and pollen of male flowers from Hippophae rhamnoides L. subsp. sinensis Rousi. The results showes that the total flavonoid content and antioxidant capacity of the perianth were higher than those of the pollen, and both exhibited good antioxidant capacity. The differences provide ideas for the separate development and utilization of the perianth and pollen, while the similarities offer data support for the overall development and utilization of male flowers from Hippophae rhamnoides L. subsp. sinensis Rousi resources.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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