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Multilocus sequence typing analysis of Vibrio parahaemolyticus isolated from Bohai Rim Region
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Yan-Hua JIANG1, Ting WANG1, 2, Meng QU1, Feng-Ling LI1, Ying-Ying GUO1, Wen-Jia ZHU1, Na LI1, Lian-Zhu WANG1, Lin YAO1, *
Journal of Food Safety & Quality | 2026, 17(6) : 48 - 57
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Journal of Food Safety & Quality | 2026, 17(6): 48-57
• Special Topic: Food Safety Risk Monitoring and Assessment •
Multilocus sequence typing analysis of Vibrio parahaemolyticus isolated from Bohai Rim Region
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Yan-Hua JIANG1, Ting WANG1, 2, Meng QU1, Feng-Ling LI1, Ying-Ying GUO1, Wen-Jia ZHU1, Na LI1, Lian-Zhu WANG1, Lin YAO1, *
Affiliations
  • 1.Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of Testing and Evaluation for Aquatic Product Safety and Quality, Ministry of Agriculture and Rural Affairs, Qingdao 266071, China
  • 2.College of Food Science and Engineering, Ocean University of China, Qingdao 266404, China
doi: 10.19812/j.cnki.jfsq11-5956/ts.20251020007
Outline
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Objective

To analyze the genetic diversity and evolution of Vibrio parahaemolyticus using multilocus sequence typing.

Methods

A total of 81 Vibrio parahaemolyticus strains isolated from shellfish in Bohai Rim Region were subjected to amplification and sequencing of 7 kinds of housekeeping genes (dnaE, gyrB, recA, dtdS, pntA, pyrC, tnaA). Sequence types (ST) was determined based on the sequences, and a phylogenetic tree was constructed to analyze genetic relationships.

Results

The 81 Vibrio parahaemolyticus strains comprised 71 kinds of STs, including 5 single-locus variants and 61 singletons. Among these, 51 kinds of STs were newly identified,accounting for 71.83% of the total. ST2369 and ST433 were the most frequently observed. The numbers of alleles(new alleles) are 49 for dnaE (5), 54 for gyrB (6), 47 for recA (3), 45 for dtdS (3), 33 for pntA (2), 47 for pyrC (3) and 34 for tnaA (4), respectively. Nucleotide diversity was lowest in pntA (0.00873) and highest in recA (0.02234). The proportion of polymorphic sites was highest for dtdS (14.19%) and lowest for tnaA (5.67%). The dN/dS ratios for all 7 kinds of housekeeping genes were below 1. Phylogenetic analysis revealed that strains with identical or highly similar ST clustered together, however, this clustering pattern did not necessarily correlate with the source of the strains.

Conclusion

Vibrio parahaemolyticus strains from shellfish in Bohai Rim Region exhibit high genetic diversity, and some strains possess the potential for cross-regional transmission, underscoring the importance of enhanced monitoring of this pathogen in seafood. These findings provide fundamental data for risk assessment and prevention of foodborne Vibrio parahaemolyticus in the Bohai Rim region.

Vibrio parahaemolyticus  /  multilocus sequencing typing  /  genetic diversity  /  evolutionary relationship
江艳华, 王婷, 曲梦, 李风铃, 郭莹莹, 朱文嘉, 李娜, 王联珠, 姚琳. 环渤海区副溶血性弧菌的多位点序列分型分析. 食品安全质量检测学报, 2026 , 17 (6) : 48 -57 . DOI: 10.19812/j.cnki.jfsq11-5956/ts.20251020007
Yan-Hua JIANG, Ting WANG, Meng QU, Feng-Ling LI, Ying-Ying GUO, Wen-Jia ZHU, Na LI, Lian-Zhu WANG, Lin YAO. Multilocus sequence typing analysis of Vibrio parahaemolyticus isolated from Bohai Rim Region[J]. Journal of Food Safety & Quality, 2026 , 17 (6) : 48 -57 . DOI: 10.19812/j.cnki.jfsq11-5956/ts.20251020007
Appendix
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Year 2026 volume 17 Issue 6
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Article Info
doi: 10.19812/j.cnki.jfsq11-5956/ts.20251020007
  • Receive Date：2025-10-20
  • Online Date：2026-11-05
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History
  • Received：2025-10-20
Funding
Affiliations
    1.Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of Testing and Evaluation for Aquatic Product Safety and Quality, Ministry of Agriculture and Rural Affairs, Qingdao 266071, China
    2.College of Food Science and Engineering, Ocean University of China, Qingdao 266404, China
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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