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Objective To explore the process of caffeine removal from green tea by supercritical carbon dioxide extraction. Methods Using Wuyuan green tea as raw material, supercritical carbon dioxide extraction was used to remove caffeine from green tea. Taking the caffeine removal rate as the investigation index, the effects of tea moisture content, extraction temperature, pressure, time, concentration and dosage of entrained agent on caffeine removal rate were studied by single factor experiment, and the caffeine removal process was optimized by orthogonal experiment. Results The optimal extraction process of caffeine removal in green tea was tea moisture content 40%, extraction pressure 30 MPa, extraction temperature 55 ℃, extraction time 240 min, concentration of entrainment agent (ethanol) 60%, and dosage of entrainment agent 1.2:1 (m:m). Under these conditions, the caffeine removal rate was 91.2%. Extraction pressure was the most important factor affecting caffeine removal rate, followed by extraction temperature, and extraction time had relatively little effect. Conclusion This study determines the best technology of caffeine removal in green tea by supercritical carbon dioxide extraction, which provides a certain reference for caffeine removal in green tea.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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