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Objective To establish 2 kinds of methods for the determination of 17-pentatriacontene in honey by gas chromatography-flame ionization detection (GC-FID) and gas chromatography-mass spectrometry (GC-MS). Methods After dissolved in water, the sample was extracted with petroleum ether, purified on a florisil cartridge, separated by DB-5MS column (15 m×0.25 mm, 0.25 μm), and determined by GC-FID and GC-MS, then quantified by external standard method. Results The GC-FID and GC-MS were quantified using matrix-free standard curve and matrix-matched standard curve respectively, with limits of quantification of 1.0 mg/kg and 0.5 mg/kg, linear ranges of 1-100 mg/kg and 0.5-40.0 mg/kg, and recovery rates ranging from 88.9% to 94.5% and from 91.2% to 98.2%, respectively. The relative standard deviations were 4.0%-6.4% and 4.6%-7.3%, respectively. The results of the 2 kinds of methods were basically consistent, with a relative deviation of no more than 2.4%. The proposed methods were applied to 158 honey samples of Apis cerana honey and Apis mellifera honey, 17-pentatriacontene was only detected in Apis cerana honey, with a content range of 0.58-51.10 mg/kg. Conclusion The accuracy and precision of the GC-FID and GC-MS methods established in this study are both good, and the 2 kinds of methods can be applied to the determination of 17-pentatriacontene in honey. The results of real sample tested indicate that 17-pentatriacontene can be used as a characteristic marker for Apis cerana honey.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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