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Objective To investigate the effects of ultrasound-assisted enzymatic hydrolysis on the sensory properties and metabolomics profiles of Dosidicus gigas viscera. Methods Three experimental groups were established: Autolytic group (B), exogenous enzymatic hydrolysis group (C) and ultrasound-assisted exogenous enzymatic hydrolysis group (U). Sensory evaluation, amino nitrogen content determination, and non-targeted metabolomics analysis were conducted to systematically compare the protein conversion efficiency under different hydrolysis strategies. Results Ultrasonic pretreatment significantly improved hydrolysis efficiency. The umami score and amino nitrogen content [(0.12±0.00) g/100 mL] of the hydrolysate in the ultrasound-assisted group (U) surpassed those of the conventional exogenous enzymatic group (C). Non-targeted metabolomics showed that the metabolites in the ultrasound-assisted exogenous enzymatic hydrolysis group (U) and the self-enzymatic hydrolysis group (B) were significantly different, and the number of differential metabolites was greater than that in the conventional exogenous enzymatic hydrolysis group (C). Primarily enriched in pathways related to plant secondary metabolite biosynthesis and ABC transporters. These findings indicated that ultrasound pretreatment enhanced enzymatic efficiency by altering protein conformation and metabolic networks. Conclusion Ultrasound-assisted enzymatic hydrolysis effectively improves the sensory characteristics of Dosidicus gigas viscera, promotes protein degradation and metabolite production, and enhances the high-value utilization and industrial production efficiency of Dosidicus gigas by-products. This study provides theoretical support and technical guidance for optimizing the processing of aquatic by-products.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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