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Detection of lipopolysaccharide using metal-organic framework-supported platinum nanoparticle-labeled immunoassay
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Jia-Yi FAN, Lin WEI, Ming-Xue ZHU, Li-Min CAO, Kai-Qiang WANG, Xiu-Dan WANG, Jian-Xin SUI*
Journal of Food Safety & Quality | 2025, 16(12) : 106 - 115
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Journal of Food Safety & Quality | 2025, 16(12): 106-115
• Highlight: Analysis and Monitoring of Toxic and Harmful Substances in Food •
Detection of lipopolysaccharide using metal-organic framework-supported platinum nanoparticle-labeled immunoassay
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Jia-Yi FAN, Lin WEI, Ming-Xue ZHU, Li-Min CAO, Kai-Qiang WANG, Xiu-Dan WANG, Jian-Xin SUI*
Affiliations
  • College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China
Published: 2025-06-25 doi: 10.19812/j.cnki.jfsq11-5956/ts.20250312006
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Objective To detection of lipopolysaccharide (LPS) by platinum nanoparticle labeling immunoassay based on metal-organic framework. Methods LPS polyclonal antibodies were obtained through immunization of mice with outer membrane vesicles (OMVs). A composite nanozyme consisting of platinum nanoparticles (Pt NPs) loaded on metal-organic framework (MIL101-NH2) was prepared as a signal label. This nanozyme was integrated with enzyme-linked immunosorbent assay (ELISA) to establish a novel immunosensing platform for LPS detection. Results The prepared LPS antibodies demonstrated a titer of 1:256000. The constructed nanozyme-based colorimetric immunoassay showed the limit of detection was 5 ng/mL (4-fold improvement in sensitivity compared with conventional enzyme-labeled ELISA), linear detection range of 20-2000 ng/mL, and satisfactory stability and specificity. Recoveries in apple juice and beer samples ranged from 90.38% to 105.71%. Conclusion This study develop LPS antibodies exhibited high specificity and strong titer. The establish Pt@MIL101-NH2-ELISA method demonstrated enhanced sensitivity and reliability, providing a new approach for immunological detection of LPS.

lipopolysaccharide  /  outer membrane vesicles  /  metal-organic framework  /  nanozyme  /  enzyme-linked immunosorbent assay
Jia-Yi FAN, Lin WEI, Ming-Xue ZHU, Li-Min CAO, Kai-Qiang WANG, Xiu-Dan WANG, Jian-Xin SUI. Detection of lipopolysaccharide using metal-organic framework-supported platinum nanoparticle-labeled immunoassay[J]. Journal of Food Safety & Quality, 2025 , 16 (12) : 106 -115 . DOI: 10.19812/j.cnki.jfsq11-5956/ts.20250312006
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Year 2025 volume 16 Issue 12
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doi: 10.19812/j.cnki.jfsq11-5956/ts.20250312006
  • Receive Date：2025-03-12
  • Online Date：2026-01-13
  • Published：2025-06-25
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  • Received：2025-03-12
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    College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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