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Objective To establish a rapid analytical protocol for the detection of multiple pesticide residues in fruits and vegetables on bioluminescence inhibition of enzyme receptor. Methods Regarding sensitivity as the index of investigating, the effects of the extraction solvent and reaction time were separately investigated. The pretreatment protocol and detection conditions parameters were optimized. Then, the sensitivity, false positive rate, false negative rate and cross-reactivity of the method were evaluated. Results The findings demonstrated that the enzyme receptor bioluminescence technique was capable of concurrently identifying several pesticide residues within fruits and vegetables, with a sensitivity that complies with the GB 2763—2021 National food safety standards-Maximum residue limits of pesticides in food. The false negative rate was 0%, the false positive rate was less than 5%, and the cross-reactivity rate was less than or equal to 0.10%. Comparative analysis utilizing both the bioluminescence inhibition of enzyme receptor and the conventional national standard method was conducted on fruit and vegetable samples sourced from the market, yielding congruent results. Conclusion The bioluminescence inhibition of enzyme receptor established in the present study is deemed suitable for the rapid assessment of pesticide residues in fruits and vegetables. It is appropriated for oversight by local regulatory bodies and assists companies in improving their internal quality control, which is highly important for boosting the quick detection ability of pesticide residues.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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