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Rapid detection of viable Pediococcus pentosaceus in rose jam by propidium monoazide-quantitative real-time polymerase chain reaction method
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Yan-Zhen ZHANG1, 2, Wei-Jie YUAN1, Chao MA1, Li-Dan MA3, Yun-Guo LIU1, *
Journal of Food Safety & Quality | 2025, 16(13) : 214 - 219
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Journal of Food Safety & Quality | 2025, 16(13): 214-219
• Food Analysis and Detection •
Rapid detection of viable Pediococcus pentosaceus in rose jam by propidium monoazide-quantitative real-time polymerase chain reaction method
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Yan-Zhen ZHANG1, 2, Wei-Jie YUAN1, Chao MA1, Li-Dan MA3, Yun-Guo LIU1, *
Affiliations
  • 1 College of Life Sciences, Linyi University, Linyi 276005, China
  • 2 College of Engineering, University of Suwon, Suwon 18323, Korea
  • 3 Gongbei Customs Technical Center, Zhuhai 519075, China
Published: 2025-07-15 doi: 10.19812/j.cnki.jfsq11-5956/ts.20250110007
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Objective To establish a rapid detection method of viable Pediococcus pentosaceus in rose jam based on the combination of propidium monoazide (PMA) and quantitative real-time polymerase chain reaction (qPCR). Methods Primers specific for Pediococcus pentosaceus were designed according to the species-specific murI gene. The optimal PMA treatment conditions were determined by optimizing the PMA concentration, dark incubation time, and exposure time. Different ratios of Pediococcus pentosaceus dead/live bacterial mixtures were validated by the PMA-qPCR method to assess the sensitivity and limit of detection of the technique. Results The designed primers exhibited high specificity, and the optimal PMA treatment conditions were determined as 80 µg/mL PMA, 15 min of dark incubation, and 25 min of exposure. Under this condition, a standard curve with correlation coefficient (r2) of 0.9983 was constructed, and the lowest limit of detection was determined to be 3.98×103 CFU/g. Conclusion The PMA-qPCR method established in this study enables the rapid and accurate quantification of viable Pediococcus pentosaceus in rose jam with high sensitivity, providing technical support for the quantitative detection of microorganisms in complex food matrices.

rose jam  /  Pediococcus pentosaceus  /  propidium monoazide  /  quantitative real-time polymerase chain reaction  /  viable
Yan-Zhen ZHANG, Wei-Jie YUAN, Chao MA, Li-Dan MA, Yun-Guo LIU. Rapid detection of viable Pediococcus pentosaceus in rose jam by propidium monoazide-quantitative real-time polymerase chain reaction method[J]. Journal of Food Safety & Quality, 2025 , 16 (13) : 214 -219 . DOI: 10.19812/j.cnki.jfsq11-5956/ts.20250110007
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Year 2025 volume 16 Issue 13
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doi: 10.19812/j.cnki.jfsq11-5956/ts.20250110007
  • Receive Date：2025-01-10
  • Online Date：2026-01-12
  • Published：2025-07-15
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  • Received：2025-01-10
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Affiliations
    1 College of Life Sciences, Linyi University, Linyi 276005, China
    2 College of Engineering, University of Suwon, Suwon 18323, Korea
    3 Gongbei Customs Technical Center, Zhuhai 519075, China
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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