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Objective To establish a method for the determination of 8 kinds of benzodiazepines residues, including diazepam, oxazepam, midazolam, clonazepam, lorazepam, triazolam, alprazolam and nitrazepam in aquatic products by ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). Methods The sample was extracted by 80% acetonitrile aqueous solution containing 0.2% formic acid, purified by Waters PRIME HLB solid phase extraction column, concentrated by nitrogen blowing in water bath. The residue contents were separated by C18 chromatographic column, eluted by 0.1% formic acid aqueous solution-methanol mobile phase systems, and analyzed by electrospray ion source in positive ion multi-reaction monitoring mode. The quantitative information was obtained by matrix-added calibration with external standard. Results The correlation coefficient of calibration curves of 8 kinds of benzodiazepines residues were above 0.999. The limits of detection were 0.064-0.226 μg/kg, the recovery rates of the method were 70.4%-107.0%, and the relative standard deviations were 2.1%-10.2%. Conclusion The method is rapid, simple and accurate, and it is suitable for the determination of benzodiazepine sedative drug residues in aquatic products.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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