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Detection of Oncorhynchus mykiss source components based on improved pre-amplification real-time quantitative polymerase chain reaction method
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Duo-Lan BAI1, Lin HONG2, Di YANG2, Wen-Chao CHEN2, Jia-Qi WANG2, Lei FANG1, Yan-Hong LIU2, *, Qiu-Yue ZHENG1, *
Journal of Food Safety & Quality | 2025, 16(13) : 18 - 25
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Journal of Food Safety & Quality | 2025, 16(13): 18-25
• Highlight: Processing and Quality Safety of Aquatic Products •
Detection of Oncorhynchus mykiss source components based on improved pre-amplification real-time quantitative polymerase chain reaction method
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Duo-Lan BAI1, Lin HONG2, Di YANG2, Wen-Chao CHEN2, Jia-Qi WANG2, Lei FANG1, Yan-Hong LIU2, *, Qiu-Yue ZHENG1, *
Affiliations
  • 1 Key Laboratory of Biotechnology and Bioresources Utilization of Ministry of Education, College of Life Science, Dalian Minzu University, Dalian 116600, China
  • 2 Dalian Center for Certification and Food and Drug Control, Technology Innovation Center of Food Safety Technique of Inspection for State Market Regulation (Rapid Screening and Traceability for Edible Agricultural Product Safety), Dalian 116630, China
Published: 2025-07-15 doi: 10.19812/j.cnki.jfsq11-5956/ts.20250415003
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Objective To establish a pre-amplified quantitative real-time polymerase chain reaction (qPCR) method for the detection of Oncorhynchus mykiss source components. Methods The study developed an enhanced pre-amplified qPCR method, which was based on the validation and improvement of the existing standard qPCR method and compared the performance of both methods. Results Specificity testing of the standard qPCR method revealed cross-reactivity with closely related species, such as Coho salmon and Atlantic salmon. The established improved pre-amplified qPCR method, which incorporated a pre-amplification step, exhibited high specificity and no cross amplification was observed for Coho salmon, Atlantic salmon and other closely related species. The amplification efficiencies of the standard qPCR and the pre-amplified qPCR methods were 90.25% and 104.91%, respectively. The lowest limits of detection of the 2 kinds of methods were 2.99×10-3 ng/μL (0.83-8.87 pg/μL, 95% confidence interval) for the standard qPCR and 1.50×10-4 ng/μL (0.09-0.27 pg/μL, 95% confidence interval) for the pre-amplified qPCR. Conclusion The method established in this study has the characteristics of strong specificity, high sensitivity, and is suitable for authenticity and adulteration detection of Oncorhynchus mykiss source components in food products.

Oncorhynchus mykiss source  /  authenticity identification  /  quantitative real-time polymerase chain reaction  /  pre-amplified quantitative real-time polymerase chain reaction
Duo-Lan BAI, Lin HONG, Di YANG, Wen-Chao CHEN, Jia-Qi WANG, Lei FANG, Yan-Hong LIU, Qiu-Yue ZHENG. Detection of Oncorhynchus mykiss source components based on improved pre-amplification real-time quantitative polymerase chain reaction method[J]. Journal of Food Safety & Quality, 2025 , 16 (13) : 18 -25 . DOI: 10.19812/j.cnki.jfsq11-5956/ts.20250415003
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Year 2025 volume 16 Issue 13
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doi: 10.19812/j.cnki.jfsq11-5956/ts.20250415003
  • Receive Date：2025-04-15
  • Online Date：2026-01-12
  • Published：2025-07-15
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  • Received：2025-04-15
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Affiliations
    1 Key Laboratory of Biotechnology and Bioresources Utilization of Ministry of Education, College of Life Science, Dalian Minzu University, Dalian 116600, China
    2 Dalian Center for Certification and Food and Drug Control, Technology Innovation Center of Food Safety Technique of Inspection for State Market Regulation (Rapid Screening and Traceability for Edible Agricultural Product Safety), Dalian 116630, China
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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