PDF
Full
Outline
Objective To optimize the enzyme-assisted extraction process of Eucommia ulmoides leaf extract, and evaluate its antioxidant and hepatoprotective activities. Methods Using Eucommia ulmoides leaves as raw material, the enzyme-assisted extraction technique was employed to optimize the extraction process. The antioxidant activity of the extract was evaluated through 1,1-diphenyl-2-picrylhydrazine (DPPH) radical scavenging rate, hydroxyl radical scavenging rate, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging rate, and total antioxidant capacity assays. The hepatoprotective activity was assessed using a zebrafish model. Results Under the conditions of a solid-to-liquid ratio of 1:20 (g:mL), enzymolysis temperature of 50 °C, enzymolysis time of 4 h, and enzyme dosage of 3%, the yield of Eucommia ulmoides leaf extract was 32.15%±0.36%, with a theoretical yield of 31.781%. The small difference between the actual and theoretical yields indicates the reliability of the extraction parameters. In vitro antioxidant activity tests showed that, within the experimental mass concentration range, the extract exhibited significant antioxidant activity, effectively scavenging DPPH radicals, hydroxyl radicals, and ABTS radicals. Zebrafish experiments demonstrated that the extract at mass concentrations of 100 μg/mL and 200 μg/mL protected against thioacetamide (TAA)-induced liver injury, indicating notable hepatoprotective activity. Conclusion The Eucommia ulmoides leaf extract obtained through enzyme-assisted extraction possesses excellent antioxidant and hepatoprotective activities. This study provides technical support for the further application and promotion of Eucommia ulmoides leaves in the food field.
PDF
253
100
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
关闭全屏
No. 86 Xueyuan South Road, Haidian District, Beijing
010-62199257
qkjq@cast.org.cn
Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House
