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Objective To construct a carbon nanotube-poly(neutral red) modified electrode for the competitive electrochemical rapid detection of putrescine in Procambarus clarkii samples. Methods Putrescine was selected as a freshness indicator for Procambarus clarkii. A carbon nanotube-poly(neutral red) modified electrode was designed for putrescine detection. The relationship between the electrochemical behavior of the electrode and the concentration of putrescine was explored, and its application in electrochemical sensing of putrescine was investigated. After optimizing factors such as scan rate and electrolyte pH, the putrescine content in Procambarus clarkii was determined. Recovery experiments were conducted, and the results were compared with high performance liquid chromatography. Results The carbon nanotube-poly(neutral red) modified electrode exhibited a significant electrochemical inhibition effect towards putrescine, with a notable inverse relationship between the concentration of putrescine and the peak current of the electrode. The linear range was described by the equation ΔI=3.8ln[c(PUT)]+8.3, with a correlation coefficient of r=0.99. When applied to the detection of putrescine in crayfish samples, the results obtained by this method were highly consistent with those obtained by the high performance liquid chromatography. Conclusion The method can be employed for the detection of putrescine concentration in Procambarus clarkii and serves as a biosensor for putrescine, a freshness indicator of Procambarus clarkii.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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