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Objective To establish a method for the determination of 9 kinds of organic acids (oxalic acid, tartaric acid, quinic acid, malic acid, lactic acid, citric acid, fumaric acid, succinic acid and gallic acid) in fruits and vegetable crisps by liquid chromatography-high resolution mass spectrometry (LC-HRMS). Methods The samples were cleaned up by a SAX solid-phase extraction column. The samples were eluted with 0.1% formic acid in water and methanol as mobile phases and separated on an Eclipse Plus C18 column (4.6 mm×250 mm, 5 μm), and monitored in electron spray ionization (ESI)- mode and quantified by external standard method. Results The calibration curves for 9 kinds of organic acids showed good linearity in the concentration range of 50-5000 ng/mL. The correlation coefficients (r) were all greater than 0.9991, the limits of detection (LODs) were in the range of 1-10 ng/mL, the limits of quantitation (LOQs) were in the range of 10-50 ng/mL, the spiked recoveries were in the range of 96%-98%, and the relative standard deviations (RSDs) were in the range of 1.5%-4.9%. Among them, lactic acid [(16.16±0.30) mg/g] and citric acid [(15.53±0.11) mg/g] were the main organic acids in the fruit and vegetable crisps, and their content was significantly higher than those of other organic acids. In contrast, tartaric acid [(1.29±0.11) mg/g] and oxalic acid [(1.56±0.25) mg/g] were found at lower levels. Conclusion The method is simple, rapid, and accurate and can be used for the qualitative and quantitative determination of organic acids (oxalic acid, tartaric acid, quinic acid, malic acid, lactic acid, citric acid, fumaric acid, succinic acid and gallic acid) in fruit and vegetable crumbles as well as other food.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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