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Fluorescent aptamer sensors based on MXene materials and X-type DNA for the detection of Pb2+ and Cd2+
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Tian BAI1, Jia-Yi SUN2, Rui-Shu JIA2, Zhi-Guang SUO2, *, Min WEI2, Hua-Li JIN2
Journal of Food Safety & Quality | 2025, 16(15) : 101 - 109
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Journal of Food Safety & Quality | 2025, 16(15): 101-109
• Special Topic: Application of Biosensors in Food Safety Detection •
Fluorescent aptamer sensors based on MXene materials and X-type DNA for the detection of Pb2+ and Cd2+
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Tian BAI1, Jia-Yi SUN2, Rui-Shu JIA2, Zhi-Guang SUO2, *, Min WEI2, Hua-Li JIN2
Affiliations
  • 1 Henan Institute of Food and Salt Industry Inspection Technology, Zhengzhou 450000, China
  • 2 College of Food Science and Engineering, Henan University of Technology, Zhengzhou 450001, China
Published: 2025-08-15 doi: 10.19812/j.cnki.jfsq11-5956/ts.20250227002
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Objective To construct a novel fluorescent aptamer sensor based on MXene nanomaterials by combining X-type DNA with aptamers for the simultaneous detection of Pb2+ and Cd2+ in food. Methods X-type DNA structures were prepared by complementary base pairing, and in the presence of Pb2+ and Cd2+, the aptamer within the X-type DNA structure specifically bound to the target ions and triggers desorption from the MXene surface, thereby restoring the fluorescent signal. Results Under the optimal experimental conditions, the fluorescence signal intensity was linearly correlated with Pb2+ and Cd2+ in the range of 0.5-500.0 nmol/L, with the limits of detection were 0.33 nmol/L and 0.28 nmol/L, respectively, and the results showed that the sensor had good working performance. Conclusion The fluorescent biosensor prepared in this study has been successfully applied to the spiked analysis of tap water and cornmeal with good recoveries, indicating that it can be used as an effective tool for the detection of Pb2+ and Cd2+ in food.

MXene nanomaterials  /  X-type DNA nanostructures  /  fluorescent biosensors  /  heavy metals
Tian BAI, Jia-Yi SUN, Rui-Shu JIA, Zhi-Guang SUO, Min WEI, Hua-Li JIN. Fluorescent aptamer sensors based on MXene materials and X-type DNA for the detection of Pb2+ and Cd2+[J]. Journal of Food Safety & Quality, 2025 , 16 (15) : 101 -109 . DOI: 10.19812/j.cnki.jfsq11-5956/ts.20250227002
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Year 2025 volume 16 Issue 15
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doi: 10.19812/j.cnki.jfsq11-5956/ts.20250227002
  • Receive Date：2025-02-27
  • Online Date：2026-01-09
  • Published：2025-08-15
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  • Received：2025-02-27
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    1 Henan Institute of Food and Salt Industry Inspection Technology, Zhengzhou 450000, China
    2 College of Food Science and Engineering, Henan University of Technology, Zhengzhou 450001, China
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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