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Objective To establish spectrophotometric method for the rapid determination of the content of 4 kinds of polyethylene nonionic surfactants. Methods The complex formed by the reaction between polyethylene-type nonionic surfactant and iodine solution exhibits corresponding light absorption at a specific wavelength. Based on the direct proportionality between the absorbance value and the concentration of the polyethylene-type nonionic surfactant, this relationship can be utilized for quantitative determination. The influences of absorption wavelength, temperature, time and other factors on the determination of nonionic surfactants were studied to achieve the best reaction conditions. Results The detection conditions were: Wavelength at 525 nm, 10 min and 25 ℃. Good linearity was observed in the range 0-30 mg/L, and the correlation coefficients were ranged from 0.9962 to 0.9988. The limits of detection were ranged from 0.0012 to 0.0022 mg/L. The relative standard deviations of samples were ranged from 0.9% to 5.1%, and the spiked recoveries were ranged from 87.2% to 91.7%. Conclusion The method is simple, accurate and credible, which is suitable for rapid detection of polyethoxylated nonionic surfactants.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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